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HyperSep Retain-CX 三聚氰胺處理SPE柱
【產(chǎn)品簡介】
【詳細(xì)說明】
HyperSep Retain-CX 三聚氰胺處理
#60107-303 RMB 1251.00
HYPERSEP Retain-CX 60MG/3ML SPE Column, PK/50
產(chǎn)品簡介:
三聚氰胺前處理
HyperSep Retain-CX 60mg/3mL
前處理:取5g樣品加入50mL 0.1%的三氯乙酸溶液中,振蕩1分鐘。加入2mL 2%醋酸鉛溶液,超聲20分鐘,轉(zhuǎn)移至10mL離心管中,以8000rpm離心10分鐘。取3mL上清液。
活化:3mL甲醇,3mL去離子水
上樣:以1-2mL/min的流速上樣
淋洗:2mL去離子水,3mL甲醇,將小柱抽干
洗脫:5mL 5%稀氨水的甲醇溶液
濃縮:50℃氮?dú)獯蹈伞?/span>2mL 20%甲醇水溶液再溶解
HyperSep Retain-CX 60mg/3mL
前處理:取5g樣品加入50mL 0.1%的三氯乙酸溶液中,振蕩1分鐘。加入2mL 2%醋酸鉛溶液,超聲20分鐘,轉(zhuǎn)移至10mL離心管中,以8000rpm離心10分鐘。取3mL上清液。
活化:3mL甲醇,3mL去離子水
上樣:以1-2mL/min的流速上樣
淋洗:2mL去離子水,3mL甲醇,將小柱抽干
洗脫:5mL 5%稀氨水的甲醇溶液
濃縮:50℃氮?dú)獯蹈伞?/span>2mL 20%甲醇水溶液再溶解
Generic Procedure for HyperSep Retain-CX:
1. Conditioning: Methanol followed by de-ionised water
2. Sample Loading: Load sample at a rate of 1-2mL/min.
3. Washing: Methanol / de-ionised water (5/95, v/v)
4. Elution: Organic solvent such as methanol or acetonitrile
Application Example – Melamine in Animal Feeds
Part Number: 60107-303
Phase: HyperSep Retain-CX
Volume: 3 mL
Bed Weight: 60 mg
Sample Pretreatment: To 5 g of animal feed, add 50 mL of 0.1% trichloroacetic acid aqueous solution. Vortex for 1 min, then add 2 mL of 2% lead acetate aqueous solution. Sonicate for 20 mins, then transfer a portion of the mixture to a 10 mL Centrifuge tube. Centrifuge at 8000 rpm for 10 mins.
Conditioning: 3 mL methanol followed by 3 mL deionized water
Application: Load 3 mL sample at 1-2 mL/min
Washing: 3 mL water followed by 3 mL methanol. Purge column to dryness
Elution: 5 mL ammonia in methanol (5:95, v/v). Dry sample under nitrogen stream. Reconstitute with 20% methanol aqueous solution.