請(qǐng)輸入產(chǎn)品關(guān)鍵字:
郵編:200000
聯(lián)系人:王小姐
電話:021-59989018
傳真:
手機(jī):15201736385
售后電話:15201736385
留言:發(fā)送留言
個(gè)性化:www.shyskits.com
網(wǎng)址:www.shyskits.com
商鋪:http://www.king-666.com/st175729/
增強(qiáng)化學(xué)發(fā)光酶免疫法
點(diǎn)擊次數(shù):3305 發(fā)布時(shí)間:2011-3-2
進(jìn)一步提高血清促甲狀腺激素(TSH)檢測(cè)的敏感性
增強(qiáng)化學(xué)發(fā)光酶免疫法測(cè)定血清促甲狀腺激素方法的建立及初步應(yīng)用
通過(guò)正交試驗(yàn),并經(jīng)方差分析,獲得增強(qiáng)發(fā)光酶免疫檢測(cè)法的*實(shí)驗(yàn)條件,將其與TSH-酶聯(lián)免疫吸附測(cè)定(ELISA)試劑盒相結(jié)合,建立TSH的增強(qiáng)化學(xué)發(fā)光酶免疫檢測(cè)法
To improve the sensitivity of measurement of TSH in serum.Methods Establishment and application of a new chemiluminescence enzyme immunoassay to determine human thyrotropin in serum LIANG Yan,LIN Hanhua,WANG Muti. Department of Pediatrics, Tongji Hospital,Tongji Medical University
The authors developed a new enhanced chemiluminescence enzyme immunoassay (ECLIA), which combined luminol-H2O2-HRP system enhanced by para-iodophenol(PIP) with TSH-ELISA test kit. The optimal experimental conditions of the enhanced chemiluminescence were obtained by orthogonal design and analysis of variance
材料和方法
儀器和試劑:Metrohm Herisau E350B-pH計(jì)(瑞士)。魯米諾 (Luminol,Serva) 用pH 10.3、0.05 mol/L 碳酸鹽緩沖液配制成5×10-2 mol/L儲(chǔ)備液放置3天后再以pH 8.2、0.1 mol/L 碳酸氫鈉-氫氧化鈉緩沖液稀釋備用。
將化學(xué)發(fā)光技術(shù)與TSH-ELISA試劑盒相結(jié)合,建立了TSH的增強(qiáng)化學(xué)發(fā)光酶免疫檢測(cè)法