WEHI-231 小鼠B淋巴細胞

WEHI-231 (ATCC® CRL-1702™) 
Organism  Mus musculus, mouse  
Cell Type  B lymphocyte, immature  
Product Format  frozen  
Morphology  lymphoblast  
Culture Properties  suspension, multicell aggregates  
Biosafety Level  1  
Disease  B cell lymphoma  
Strain  (BALB/c x NZB)F1  WEHI-231 小鼠B淋巴細胞
Applications  This cell line is a suitable transfection host.  
Storage Conditions  liquid nitrogen vapor phase  
Images    
Derivation  Mineral oil induced tumor in (BALB/c x NZB)F1 mice  
Genes Expressed  immunoglobulin 
Comments  WEHI-231 cells do not secrete IgM into medium unless stimulated with lipopolysaccharide (0.01 to 3 μg/mL). Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium  The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%. 
 
Subculturing  Optimum culture recovery from a frozen vial is in a T25 flask at a density between 2 to 5 X 105 viable cells/mL. If recovered at 5 X 105 viable cells/mL, media will need to be added to the culture within the first 3 days, before the density reaches 1 X 106 viable cells/mL, at which point the viability drops drastically. 
After cells recover, expansions can be done at 1 X 105 viable cells/mL by adding media to decrease the cell concentration.
This cell line forms tight clusters of viable cells accompanied by some single non-viable cells plus debris in suspension. With increased growth the clusters become larger. As the cell density nears 1 X 106 cells/mL, the single (non-clustered) and non-viable cells increase along with the amount of debris.
At subculture, break up clusters by gentle pipetting.
Established cultures can be grown in T75 flasks.
Subc*tion Ratio: Add fresh medium every 2 to 3 days (depending on cell density)
Medium Renewal: Every 2 to 3 days 
Cryopreservation  Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase 
Culture Conditions  Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C

Isotype  IgM , IgM (surface)  
Population Doubling Time  about 20 hours  
Name of Depositor  NL Warner, LL Lanier  
References  Boyd AW, et al. The regulation of growth and differentiation of a murine B cell lymphoma. I. Lipopolysaccharide induced differentiation. J. Immunol. 126: 2461-2465, 1981. PubMed: 6785355

Lanier LL, Warner NL. Cell cycle related heterogeneity of Ia antigen expression on a murine B lymphoma cell line:analysis by flow cytometry. J. Immunol. 126: 626-631, 1981. PubMed: 6969756  

Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803

mineral oil induced tumor in (BALB/c x NZB)F1 mice

To secrete IgM into the medium, these cells need to be stimulated with lipopolysaccharide (0.01 to 3 mcg/ml). 

References  Boyd AW, et al. The regulation of growth and differentiation of a murine B cell lymphoma. I. Lipopolysaccharide induced differentiation. J. Immunol. 126: 2461-2465, 1981. PubMed: 6785355

Lanier LL, Warner NL. Cell cycle related heterogeneity of Ia antigen expression on a murine B lymphoma cell line:analysis by flow cytometry. J. Immunol. 126: 626-631, 1981. PubMed: 6969756

Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803

mineral oil induced tumor in (BALB/c x NZB)F1 mice

To secrete IgM into the medium, these cells need to be stimulated with lipopolysaccharide (0.01 to 3 mcg/ml). 

MLE-12 小鼠肺上皮細胞
saos-2 人成骨肉瘤細胞
MA104 非洲綠猴胚胎腎上皮細胞
ball-1 細胞
nci-h226 人肺鱗癌細胞
MCA38 小鼠結(jié)腸癌細胞
LTPA 小鼠胰腺癌細胞 
NCI-H23 人非小細胞肺癌細胞
293.00     人胚腎細胞

293FT    人胚腎細胞-用于慢病毒包裝
293TN    人胚腎細胞--用于慢病毒包裝
293AV    人胚腎細胞-用于腺病毒包裝
293T    人胚腎細胞
208F    大鼠成纖維細胞
786-O    人腎透明細胞腺癌細胞
A172    人腦膠質(zhì)母細胞瘤細胞
A549    人肺癌細胞 細胞系購買
AGS    人胃癌細胞
BEL7402 人肝癌細胞系
AZ-521    人胃癌細胞
B16F0    小鼠黑色素瘤細胞
Bel-7402    人肝癌細胞
Bel-7404    人肝癌細胞
BGC-823    人胃癌細胞
C6    大鼠腦膠質(zhì)瘤細胞
SW480    人結(jié)腸腺癌細胞
CaCo-2    人結(jié)腸癌細胞
Cho-K1    倉鼠卵巢細胞亞株
COS-7    非洲綠猴SV40轉(zhuǎn)化的腎細胞
GES-1    人胃上皮細胞
HCT-8    人回盲腸癌細胞
HeLa    人宮頸癌細胞
Hepa1-6    小鼠肝癌細胞
HepG2    人肝癌細胞
hFOB1.19    人永生化成骨細胞
HGC-27    人胃癌細胞
Hs 746T    人胃癌細胞
HT-29    人結(jié)腸癌細胞
KB    人口腔表皮樣癌細胞
LoVo    人結(jié)腸癌細胞
LLC-PK1    豬腎近曲小管上皮細胞
MCF-10A    人正常乳腺上皮細胞
MCF-7    人乳腺癌細胞
MDA-MB-231    人乳腺癌細胞
MGC80-3    人胃癌細胞
MKN-45    人胃癌細胞
NCI-H460    人大細胞肺癌細胞
NIH-3T3    小鼠成纖維細胞
NUGC-3    人胃癌細胞
Raw264-7    小鼠單核細胞白血病細胞
SGC-7901    人胃癌細胞
SMMC-7721    人肝癌細胞
SNU-638    人胃癌細胞
SNU-739    人肝癌細胞
Tca8113    人舌鱗癌細胞    細胞系購買
U251    人腦膠質(zhì)瘤細胞
U-373MG    人腦神經(jīng)膠質(zhì)瘤細胞
U-87 MG    人腦星形膠質(zhì)母細胞瘤
BT-474    人乳腺導(dǎo)管癌細胞
PANC-1    人胰腺癌細胞
HEK293    人胚腎細胞
Hep3B    人肝癌細胞
HL-7702    人正常肝細胞
Raw-Blue    小鼠單核細胞白血病細胞
WEHI-231    小鼠B淋巴細胞
OPM-2    人骨髓瘤細胞
NCI-H929    人骨髓瘤細胞

U266    人骨髓瘤細胞
EJ    人膀胱癌細胞
HOC    大鼠肝卵圓細胞
RWPE-1    人正常前列腺上皮細胞
CAL 27    人舌鱗癌細胞
Calu-3    人肺腺癌細胞
Eca-109    人食管癌細胞
DLD-1    人結(jié)直腸腺癌上皮細胞
5637.00     人膀胱癌細胞
SK-OV-3    人卵巢癌細胞
GBC-SD    人膽囊癌細胞
BEL7402 人肝癌細胞系
SW620    人結(jié)腸癌細胞
MRC-5    人胚肺成纖維細胞     細胞系購買
PC9    人肺癌細胞
PC14    人肺癌細胞
BxPC    人原位胰腺腺癌細胞
PK（15）    豬腎上皮細胞
MDCK    狗腎上皮細胞
3D4/21    豬肺泡巨噬細胞

MHCC-97L    人肝癌細胞（低轉(zhuǎn)移）
MHCC-97H    人肝癌細胞系（高轉(zhuǎn)移）
MiaCaPa-2    人胰腺癌細胞
HN13    口腔鱗狀細胞癌細胞
Bend3    小鼠腦微血管內(nèi)皮細胞
QBC939    人膽管癌細胞
Ishikawa    人子宮內(nèi)膜癌細胞
A498    人腎癌細胞
143B    
WEHI-231    小鼠B淋巴細胞
BN    
P9R    小鼠骨髓基質(zhì)細胞
P9    小鼠骨髓基質(zhì)細胞
F98    大鼠腦膠質(zhì)瘤細胞
NCIH1299    人非小細胞肺癌細胞
NCIH1650-M3    人非小細胞肺癌細胞
293LVT    人胚腎細胞--用于慢病毒包裝
EC9706    人食管癌細胞
CNE    人鼻咽癌細胞
H322    人非小細胞肺癌細胞
Panc O2    
S8    BEL7402 人肝癌細胞系
R5    細胞系購買
Pig MSC    豬骨髓間質(zhì)干細胞
HCAEC    人冠狀動脈內(nèi)皮細胞
marc-145    猴胚胎腎上皮細胞
3D4/21    豬肺泡巨噬細胞
PK（15）    豬腎上皮細胞
MADB-106    大鼠乳腺癌細胞
Sf9    昆蟲卵巢細胞
DADY    
HUVEC[HUV-EC-C]    人臍靜脈內(nèi)皮細胞
PC14    人肺癌細胞
PC9    人肺癌細胞
Eca-109    人食管癌細胞
CAL 27    人舌鱗癌細胞
HOC    肝卵圓細胞
EJ    人膀胱癌細胞

U266    人骨髓瘤細胞
293-mTLR5    人胚腎細胞
Raw-Blue    小鼠單核細胞白血病細胞
HL-7702    人正常肝細胞
Hep3B    人肝癌細胞
U-373MG    人腦神經(jīng)膠質(zhì)瘤細胞
Tca-8113    人舌鱗癌細胞
SNU-739    人肝癌細胞
SNU-638    人胃癌細胞
NUGC-3    人胃癌細胞
NIH-3T3    小鼠成纖維細胞
MKN-45    人胃癌細胞
LLC-PK1    豬腎近曲小管上皮細胞
Hs 746T    人胃癌細胞
Hepa1-6    小鼠肝癌細胞
HCT-8    人回盲腸癌細胞
BEL7402 人肝癌細胞系
HaCaT    人永生化表皮細胞
GES-1    人胃上皮細胞
B16-F0    小鼠黑色素瘤細胞
AZ-521    人胃癌細胞
786-O    人腎透明細胞腺癌細胞
208F    大鼠成纖維細胞
293TN    人胚腎細胞--用于慢病毒包裝
293FT    人胚腎細胞-用于慢病毒包裝
GP-293    人胚腎細胞
293-L.P.    人胚腎細胞