CV-1 非洲綠猴腎細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
CV-1 非洲綠猴腎細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
CV-1 非洲綠猴腎細(xì)胞 的詳細(xì)介紹
CV-1 非洲綠猴腎細(xì)胞
| ATCC? Number: | CCL-70? | Price: | $289.00 |
| Designations: | CV-1 | | Depositors: | FC Jensen | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Cercopithecus aethiops | | Morphology: | fibroblast
| | Source: | Organ: kidney
Disease: normal | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| | Applications: | efficacy testing [92587]
transfection host （Roche FuGENE? Transfection Reagents）
viruscide testing [92582] [92579] | | Virus Susceptibility: | poliovirus 1; herpes simplex; Eastern equine encephalitis; Western equine encephalitis; California encephalitis; SV40 | | Reverse Transcript: | negative | | Cytogenetic Analysis: | This is a pseudodiploid, male African green monkey cell line. The modal chromosome number was 60, occurring in 48% of cells, and the rate of polyploidy was at 4.4%. Only a few markers were found., Of these M1, a probable deleted N11, was found in all cells examined; M3 of unknown origin was in some cells; and the remaining 2 to 3 others of unknown origins were found only once., N11 was uniformly single copied, and N16 was also single copied in most cells. Both X and Y chromosomes were also detected in every cell. | | Age: | 141 days | | Gender: | male | | Comments: | Suitable host for transfection, especially by SV40 vectors.
The CV-1 cell line was derived from the kidney of a male adult African green monkey by F.C. Jensen, et al. in March, 1964 for use in Rous sarcoma virus transformation studies. [22138]
Derived from the kidney of male adult African green monkey. | | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C | | Subculturing: | Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. | | Preservation: | Culture medium, 95%; DMSO, 5% |
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