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Applications:
assay of NK cell activity （ [22345] [49299] The cells are sensitive to the action of natural killer （NK） cells and are useful in assays of NK cell activity.）
Comments:
Tested and found negative for ectromelia virus （mousepox）.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide （CO2）, 5% Temperature: 37.0°C
Subculturing:
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10（5） cells/ml and maintain between 2 X 10（5） and 2 X 10（6） cells/ml. Medium Renewal: Every 2 to 3 days
Preservation:
Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:
Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001 recommended serum:ATCC 30-2020 Cell culture tested DMSO:ATCC 4-X
References:
22345: Kiessling R, et al. "Natural" killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype. Eur. J. Immunol. 5: 112-117, 1975. PubMed: 1234049 26348: Cikes M, et al. Progressive loss of H-2 antigens with concomitant increase of cell- surface antigen（s） determined by Moloney leukemia virus in cultured murine lymphomas. J. Natl. Cancer Inst. 50: 347-362, 1973. PubMed: 4573851 32552: Okazaki IJ, et al. Cloning and characterization of a novel membrane-associated lymphocyte NAD:arginine ADP-ribosyltransferase. J. Biol. Chem. 271: 22052-22057, 1996. PubMed: 8703012 49299: Kiessling R, et al. "Natural" killer cells in the mouse. II. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Characteristics of the killer cell. Eur. J. Immunol. 5: 117-121, 1975. PubMed: 1086218
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