3D Cell Culture Chip三維細(xì)胞培養(yǎng)芯片
類別：細(xì)胞培養(yǎng)芯片、微流控芯片

INITIO是一家創(chuàng)新型公司，他的名字“INITIO"，來自拉丁語單詞“initium"，意思是“入口或開始"initio將細(xì)胞培養(yǎng)推向了一個新的開始，一個新的維度：芯片上的3d器官。

ddi芯片是一種*且易于使用的微流控設(shè)備，具有五個相互連接的通道，能夠?yàn)閺V泛的應(yīng)用和分析重建生物復(fù)雜的微環(huán)境。

3D Cell Culture Chip三維細(xì)胞培養(yǎng)芯片

 

ddi芯片是一種微流控器件，通過它可以在不同的距離上研究細(xì)胞和因子之間的相互作用。這提供了一個更生理的設(shè)置，并允許同時調(diào)查不同的距離在同一設(shè)備。使用DDI芯片還可以測試影響距離相關(guān)交互的代理。

 

 

材料

ddi芯片由生物相容性聚二甲基硅氧烷（pdms）制成，具有透氣性，保證了co2和o2的交換。薄玻璃載體是一種具有高透明、低自熒光等優(yōu)良光學(xué)性能的顯微鏡載玻片。這允許通過光學(xué)顯微鏡、熒光顯微鏡和共焦顯微鏡以時間和空間分辨率直接觀察生物樣品。

 

 

 

技術(shù)特點(diǎn)

模式包括：

 

2個營養(yǎng)液供應(yīng)和受試化合物注射的介質(zhì)通道（外部通道）；

2個基質(zhì)通道（中間通道），用于細(xì)胞接種或不接種水凝膠；

1主中央通道，寬度沿其長度逐漸增加

 

邊介質(zhì)通道中間矩陣通道中間通道

高度150微米150微米150微米

寬度2500微米

1125微米（狹窄部分）

 

3375微米（寬部分）

 

從1250微米逐漸變?yōu)?000微米

體積（建議注射）

 

20微升20微升20微升

入口直徑1000微米1000微米1000微米1000微米

 

DDI芯片沒有涂層表面。根據(jù)您的應(yīng)用，并終改善細(xì)胞粘附性，您可以使用聚合物和/或蛋白質(zhì)溶液來覆蓋通道表面。

 

這種微流控裝置還可以與細(xì)胞嵌入水凝膠（如I型膠原、IV型膠原、纖維蛋白、基質(zhì)凝膠等）中，以重建可靠的生物微環(huán)境。

 

入口直徑為1毫米，可通過普通吸管輕松注入細(xì)胞懸液20微升是每個通道/腔室的推薦體積；這將允許在實(shí)際注射和*填充時有少量額外的微升。

 

 

 

此外，還可以使用直接安裝在入口的1/16“管子在動態(tài)流動條件下進(jìn)行研究。

 

 

收益

使用方便

生理相關(guān)微環(huán)境

單細(xì)胞分辨率

直接可視化

時空分辨率

定性和定量評估

3D Cell Culture Chip三維細(xì)胞培養(yǎng)芯片

 

The DDI-Chip is a microfluidic device through which the interaction between cells and factors can be investigated at different distances from each other. This provides a more physiological setup and allows simultaneous investigation of different distances in the same device. With the DDI-Chip it is also possible to test agents that can affect the distance dependent interactions.

 

Materials

The DDI-Chip is made of biocompatible polydimethylsiloxane (PDMS) and is gas-permeable to ensure the CO2 and O2 exchange. The support in thin glassis a microscope slide with excellent optical properties such as high transparency and low autofluorescence. This allows a direct observation of your biological sample with temporal and spatial resolution by using optical, fluorescent and confocal microscopes.

 

 

Technical features 

The pattern consists in:

• 2 medium channels (the external ones) for nutrient supply and tested compounds injection;
• 2 matrix channels (the intermediate ones) for cell seeding with or w/o hydrogels;
• 1 main central channel with an width that increases progressively along its length 

 

	Side Medium Channels	Intermediate Matrix Channels	Middle Channel
Height	150 µm	150 µm	150 µm
Width	2500 µm	1125 µm (the narrow parts) 3375 µm (the wide parts)	Gradually changed from 1250 µm to 5000 µm
Volume (recommended for injection)	20 µL	20 µL	20 µL
Diameter of inlets	1000 µm	1000 µm	1000 µm

 

The DDI-Chip has not coated surfaces. According to your application and to eventually improve cell adhesion, you can coat the channel surface by using your solutions of polymers and/or proteins. 

This microfluidic device can be used also with cells embedded in hydrogels such as Collagen I, Collagen IV, Fibrin, Matrigel, etc. to recreate a reliable biological microenvironment. 

The inlet diameter of 1 mm allows an easy injection of the cell suspension through common pipettes. 20 µL is the recommended volume for each channel/chamber; this will allow to have few extra µL for a practical injection and complete filling.

 

 

Additionally, it would be possible to perform studies in dynamic flow conditions by using 1/16” tubing directly fitted in the inlets. 

 

 

 

Benefits

• Easy-to-use
• Physiologically relevant microenvironment 
• Single cell resolution 
• Direct visualization
• Temporal and spatial resolution 
• Qualitative and quantitative assessments