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The EpiQuik™ Tissue Acetyl-Histone H3 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3 acetylation and DNA in variety of mammalian tissues. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Tissue Acetyl-Histone H3 ChIP kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray.

WHY CHOOSE THE EPIQUIK™ TISSUE ACETYL-HISTONE H3 ChIP KIT?

• The fastest procedure available, which can be finished within 5 hours.
• Strip microwell format makes the assay flexible: manual or high throughput.
• Columns for DNA purification are included: save time and reduce labor.
• Compatible with all DNA amplification-based approaches.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Tissue Acetyl-Histone H3 ChIP kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for acetyl-histone H3 from mammalian cells. Particularly, this kit includes a ChIP-grade acetyl-histone H3 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured acetyl-histone H3 protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

 

SCHEMATIC PROCEDURE:

CP1 （Wash Buffer）
CP2 （Antibody Buffer）
CP3 （Lysis Buffer）
CP4 （ChIP Dilution Buffer）
CP5 （DNA Release Buffer）
CP6 （Reverse Buffer）
CP7 （Binding Buffer）
CP8 （Elution Buffer）
Homogenizing Buffer
Protease Inhibitor cocktail （100X）*
Proteinase K （10 mg/ml）*
Normal Mouse IgG （0.5 mg/ml）*
Anti-Acetyl-Histone H3 （1 mg/ml）*
8-Well Assay Strips （with Frame）
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by ing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

Shio, S. et. al. （January 2011）. Loss of RUNX3 expression by histone deacetylation is associated with biliary tract carcinogenesis.Cancer Sci. Epub ahead of print. PubMed Abstract