細胞型號

培養(yǎng)基

每孔細

胞數(shù)

DNA的量

轉(zhuǎn)染試劑量

和培養(yǎng)基混合 4-6h

293H

DMEM

3×10⁴

0.2µg

0.5µL

DMEM+10%FBS

293FT

DMEM

3×10⁴

0.2µg

0.5µL

DMEM+10%FBS

293E

DMEM

3×10⁴

0.2µg

0.5µL

DMEM+10%FBS

293F

DMEM

3×10⁴

0.2µg

0.5µL

DMEM+10%FBS

COS7

DMEM

1.5×10⁴

0.4µg

0.5µL

DMEM+10%FBS

hela

DMEM

2×10⁴

0.3µg

0.5µL

1640+15%FBS

Caco2

MEM

3.5×10⁴

0.3µg

0.75µL

MEM+10%FBS

BHK21

MEM

2×10⁴

0.2µg

0.5µL

MEM+10%FBS

CHO-DG44

DMEM+HT+pro

2×10⁴

0.5µg

0.5µL

DMEM+HT+pro +10%FBS

RAW264.7

DMEM

3×10⁴

0.2µg

0.5µL

DMEM +10%FBS

MCF7

MEM/NEAA+0.01mg/mL insulin + sodium pyruvat

2×10⁴

0.1µg

0.25µL

MEM/NEAA+0.01mg/mL insulin + sodium pyruvat+10%FBS

SW480

IMDM

3×10⁴

0.4µg

0.5µL

IMDM +10%FBS

MDCK

DMEM

4×10⁴

0.6µg

1µL

DMEM+10%FBS

CHO-K1

IMDM+Pro

3×10⁴

0.2µg

0.5µL

IMDM+Pro +10%FBS

HepG2

DMEM

3×10⁴

0.5µg

0.75µL

DMEM+10%FBS

A549

DMEM

2×10⁴

0.3µg

0.5µL

DMEM+10%FBS

NIH/3T3

DMEM

1.5×10⁴

0.1µg

0.75µL

DMEM+10%FBS

vero

DMEM

3×10⁴

0.3µg

0.75µL

DMEM+10%FBS

sf9

SIM SF

5×10⁴

0.4µg

0.75µL

SIM SF+10%FBS

細胞種類

中文名稱

PEI 轉(zhuǎn)染效率

HEK293

人胚腎細胞

90%-95%

293-T

人胚腎細胞

90%-95%

CHO-K1

倉鼠卵巢細胞

80%-90%

U251

人源神經(jīng)膠質(zhì)瘤細胞

80%-90%

Hela

人源宮頸癌細胞

70%-80%

COS7

猴SV40轉(zhuǎn)化腎細胞

70%-80%

HepG2

人源肝癌細胞

70%-80%

NIH/3T3

小鼠胚胎成纖維細胞

60%-70%

膠質(zhì)瘤干細胞

人源膠質(zhì)瘤干細胞

60%-70%

Patu8988

人源胰腺癌細胞

60%-70%

U2OS

人源骨肉瘤細胞

60%-70%