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上海士鋒生物關(guān)于新型顯現(xiàn)圖片方法揭示細(xì)菌與免疫系統(tǒng)作用機(jī)制
最近更新時間：2013-7-9
提 供 商：上海士鋒生物科技有限公司資料大小：14KB
文件類型：JPG 圖片下載次數(shù)：180次
資料類型：瀏覽次數(shù)：1195次
慢性感染是發(fā)達(dá)國家近些年來的一個龐大而且不斷增長的問題，當(dāng)然，相應(yīng)的慢性感染研究也在不斷進(jìn)行來研究抵御頑強(qiáng)細(xì)菌感染的方法。當(dāng)細(xì)菌許多細(xì)胞集中在一起形成生物被膜結(jié)構(gòu)時，細(xì)菌會表現(xiàn)出對抗生素*的耐藥性。截至到現(xiàn)在，科學(xué)家們緊緊能夠預(yù)測在慢性感染期間當(dāng)細(xì)菌擊敗機(jī)體免疫系統(tǒng)后所發(fā)生的反應(yīng)。
研究者Thomas解釋道，盡管我們一直懷疑細(xì)菌是如何引發(fā)慢性感染的，細(xì)菌可以破壞免疫系統(tǒng)的白細(xì)胞?，F(xiàn)在這種新型方法將使得我們更地觀察慢性感染過程中的細(xì)節(jié)。取代了觀察細(xì)菌表面結(jié)構(gòu)，目前我們可以直接觀察到細(xì)菌的截面，然后跟蹤細(xì)菌如何與白細(xì)胞以及抗生素進(jìn)行反應(yīng)，這將使我們理解慢性感染的基本過程。
Maria博士表示，這種新型方法將使得我們研究細(xì)菌分泌的化合物如何與白細(xì)胞進(jìn)行作用，白細(xì)胞可以產(chǎn)生DNA陷阱用來捕獲細(xì)菌，但是這僅僅只是一個猜測。
Interactions between Polymorphonuclear Leukocytes and Pseudomonas aeruginosa Biofilms on Silicone Implants In Vivo
Maria van Gennipa, Louise Dahl Christensena, Morten Alhedea,c, Klaus Qvortrupb, Peter Østrup Jensenc, Niels Høibya,c, Michael Givskova,d and Thomas Bjarnsholta,c
Chronic infections with Pseudomonas aeruginosa persist because the bacterium forms biofilms that are tolerant to antibiotic treatment and the host immune response. Scanning electron microscopy and confocal laser scanning microscopy were used to visualize biofilm development in vivo following intraperitoneal inoculation of mice with bacteria growing on hollow silicone tubes, as well as to examine the interaction between these bacteria and the host innate immune response. Wild-type P. aeruginosa developed biofilms within 1 day that trapped and caused visible cavities in polymorphonuclear leukocytes （PMNs）. In contrast, the number of cells of a P. aeruginosa rhlA mutant that cannot produce rhamnolipids was significantly reduced on the implants by day 1, and the bacteria were actively phagocytosed by infiltrating PMNs. In addition, we identified extracellular wire-like structures around the bacteria and PMNs, which we found to consist of DNA and other polymers. Here we present a novel method to study a pathogen-host interaction in detail. The data presented provide the first direct, high-resolution visualization of the failure of PMNs to protect against bacterial biofilms. .