| CBP30092L | |
| I. Introduction | |
| Host Cell: | A2058 (BRAF,p.V600E) |
| Expressed gene: | Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+2ug/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Skin; derived from metastatic site: lymph node |
| Disease: | Human caucasian metastatic melanoma |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| III. Representative Data | |
| |
Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System.. The number of Luminescence signal is 530±10(Mean±SD) on A2058 cells while789677±27426(Mean±SD) on A2058-Luc cells. | |
| IV. Stablity Test | |
 | |
Figure. Stablity test results. A2058-Luc cells showed at least 32 passage stability with similar luminescence unit. | |
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