| I. Background | |
集落刺激因子 1（Colony Stimulating Factor 1，CSF1），又稱巨噬細(xì)胞集落刺激因 子（M-CSF），是由兩條相同多肽鏈通過二硫鍵形成的同源二聚體，是導(dǎo)致各種炎癥性疾 病的最常見的促炎細(xì)胞因子之一。其在骨關(guān)節(jié)炎、癌癥和其他自身免疫性疾病的發(fā)展和進(jìn) 展中具有顯著的作用。 | |
| II. Desription | |
CSF1R SRE-Luc HEK293 報告基因藥靶模型很好的模擬了體內(nèi) CSF1 的信號轉(zhuǎn)導(dǎo)過程， 原理見下圖所示。
Figure 1. CSF1R SRE-Luc HEK293 細(xì)胞模型原理圖 | |
| Ⅱ. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | CFS1R-SRE-Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | FMS, CD115 |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+200 μg/ml Hygromycin B+1 μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Embryonic kidney |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
Figure 2. Recombinant CSF1R/SRE-Luc/HEK293 stably expressing CSF1R.
Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose Response of Recombinant Human CSF1 in CSF1R SRE-Luc HEK293(C2).
Figure 4. Inhibition of Human CSF1-induced Reporter Activity by CPDs in SRE-Luc HEK293(C2).
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