I. Background | |
集落刺激因子 1(Colony Stimulating Factor 1,CSF1),又稱巨噬細(xì)胞集落刺激因 子(M-CSF),是由兩條相同多肽鏈通過二硫鍵形成的同源二聚體,是導(dǎo)致各種炎癥性疾 病的最常見的促炎細(xì)胞因子之一。其在骨關(guān)節(jié)炎、癌癥和其他自身免疫性疾病的發(fā)展和進(jìn) 展中具有顯著的作用。 | |
II. Desription | |
CSF1R SRE-Luc HEK293 報告基因藥靶模型很好的模擬了體內(nèi) CSF1 的信號轉(zhuǎn)導(dǎo)過程, 原理見下圖所示。 Figure 1. CSF1R SRE-Luc HEK293 細(xì)胞模型原理圖 | |
Ⅱ. Introduction | |
Host Cell: | HEK293 |
Expressed gene: | CFS1R-SRE-Luciferase |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | FMS, CD115 |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | DMEM+10%FBS+200 μg/ml Hygromycin B+1 μg/ml Puromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
Ⅲ. Description of Host Cell Line | |
Organism: | Human |
Tissue: | Embryonic kidney |
Morphology: | Epitheloid cell |
Growth Properties: | Adherent |
Ⅳ. Representative Data |
Figure 2. Recombinant CSF1R/SRE-Luc/HEK293 stably expressing CSF1R.
Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). Dose Response of Recombinant Human CSF1 in CSF1R SRE-Luc HEK293(C2).
Figure 4. Inhibition of Human CSF1-induced Reporter Activity by CPDs in SRE-Luc HEK293(C2).