FcεRI Effector Reporter Cell
| I. Background | |
IgE由兩條重鏈（ε鏈）和兩條輕鏈組成，F(xiàn)c段含高親和力受體（FcεRI）結(jié)合位點(diǎn)，半衰期僅2-3天（IgG為21天）。作為免疫系統(tǒng)的“哨兵"，IgE既可結(jié)合寄生蟲(chóng)表面抗原，激活嗜酸性粒細(xì)胞釋放毒性顆粒（如主要堿性蛋白），通過(guò)ADCC（抗體依賴細(xì)胞介導(dǎo)的細(xì)胞毒性）清除寄生蟲(chóng)（如血吸蟲(chóng)、鉤蟲(chóng)）；也能因過(guò)度反應(yīng)引發(fā)致命過(guò)敏；IgE還可靶向腫瘤抗原（如HER2），激活巨噬細(xì)胞ADCC效應(yīng)；并且最近有研究指出，腦膜肥大細(xì)胞通過(guò)IgE-MRGPRX2可加劇阿爾茨海默病病理。 | |
| II. Description | |
FcεRI Effector Reporter Cell 藥靶模型很好的模擬了 FcεRI的信號(hào)轉(zhuǎn)導(dǎo)過(guò)程，原理見(jiàn)下圖所示。
Figure 1. FcεRI Effector Reporter Cell細(xì)胞模型原理圖 | |
| III. Introduction | |
| Expressed gene: | FcεRI |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+ 1 μg/ml Puromycin+ 10 μg/ml Blasticidin + 800 μg/ml Hygromycin B+ 1000 μg/ml G418 |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data
Figure 2. Recombinant FcεRI Effector Reporter Cell stably expressing FcεRIA.
Figure 3. Dose Response of Recombinant Human IgE in FcεRI Effector Reporter Cell(C4).
Figure 4. Inhibition of Human IgE-induced Reporter Activity by Human IgE Neutralization Ab in FcεRI Effector Reporter Cell(C4). | |
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