欧美国产日韩在线免费观看-欧美日韩成人激情一区二区-欧美久久综合一区二区-亚洲av寂寞少妇久久

| 注冊(cè)| 產(chǎn)品展廳| 收藏該商鋪

行業(yè)產(chǎn)品

當(dāng)前位置:
旭月(北京)科技有限公司>>技術(shù)文章>>JXB:西北寒旱所、蘭大丨FADs在植物脅迫耐受中的功能(鈣信號(hào))

JXB:西北寒旱所、蘭大丨FADs在植物脅迫耐受中的功能(鈣信號(hào))

閱讀:562        發(fā)布時(shí)間:2019-6-14

作者:中科院西北生態(tài)環(huán)境資源研究所石玉蘭、蘭州大學(xué)生科院安黎哲

標(biāo)題:Integrated regulation triggered by a cryophyte ω-3 desaturase gene confers multiple-stress tolerance in tobacco

主題:ω-3脂肪酸去飽和酶在植物脅迫耐受中的功能

期刊:Journal of Experimental Botany

影響因子:5.354

檢測(cè)指標(biāo):Ca2+

英文摘要

ω-3 fatty acid desaturases (FADs) are thought to contribute to plant stress tolerance mainly through linolenic acid (C18:3)-induced membrane stabilization, but a comprehensive analysis of their roles in stress adaptation is lacking.

Here, we isolated a microsomal ω-3 FAD gene (CbFAD3) from a cryophyte (Chorispora bungeana) and elucidated its functions in stress tolerance. CbFAD3, exhibiting a high identity to Arabidopsis AtFAD3, was up-regulated by abiotic stresses. Its functionality was verified by heterogonous expression in yeast. Overexpression of CbFAD3 in tobacco constitutively increased C18:3 in both leaves and roots, which maintained the membrane fluidity, and enhanced plant tolerance to cold, drought, and salt stresses.

Notably, the constitutively increased C18:3 induced a sustained activation of plasma membrane Ca2+-ATPase, thereby, changing the stress induced Ca2+ signaling. The reactive oxygen species (ROS) scavenging system, which was positively correlated with the level of C18:3, was also activated in the transgenic lines.

Microarray analysis showed that CbFAD3-overexpressing plants increased the expression of stressresponsive genes, most of which are affected by C18:3, Ca2+, or ROS. Together, CbFAD3 confers tolerance to multiple stresses in tobacco through the C18:3-induced integrated regulation of membrane, Ca2+, ROS, and stress-responsive genes. This is in contrast with previous observations that simply attribute stress tolerance to membrane stabilization.

中文摘要(谷歌機(jī)翻)

ω-3脂肪酸去飽和酶(FADs)被認(rèn)為主要通過亞麻酸(C18:3)誘導(dǎo)的膜穩(wěn)定作用促進(jìn)植物抗逆性,但缺乏對(duì)其在脅迫適應(yīng)中的作用的綜合分析。

在這里,我們從冷凍植物(Chorispora bungeana)中分離出微粒體ω-3 FAD基因(CbFAD3),并闡明其在脅迫耐受中的功能。表現(xiàn)出與擬南芥AtFAD3高度同一性的CbFAD3被非生物脅迫上調(diào)。其功能通過酵母中的異源表達(dá)來驗(yàn)證。煙草中CbFAD3的過量表達(dá)在葉和根中組成性地增加了C18:3,這保持了膜的流動(dòng)性,并增強(qiáng)了植物對(duì)冷,干旱和鹽脅迫的耐受性。

值得注意的是,組成型增加的C18:3誘導(dǎo)質(zhì)膜Ca2+-ATPase的持續(xù)活化,從而改變應(yīng)激誘導(dǎo)的Ca2+信號(hào)傳導(dǎo)。與C18:3水平正相關(guān)的活性氧(ROS)清除系統(tǒng)也在轉(zhuǎn)基因系中被激活。

微陣列分析顯示過表達(dá)CbFAD3的植物增加了應(yīng)激反應(yīng)基因的表達(dá),其中大多數(shù)受C18:3,Ca2+或ROS的影響。 CbFAD3一起通過C18:3誘導(dǎo)的膜,Ca2+,ROS和應(yīng)激反應(yīng)基因的整合調(diào)節(jié)賦予對(duì)煙草中多種脅迫的耐受性。這與先前的觀察結(jié)果形成對(duì)比,之前的觀察結(jié)果僅僅將壓力耐受性歸因于膜穩(wěn)定。

Ca2+ fluxes in root tips treated with 15% PEG or 200 mM NaCl (n=10 ten-day-old seedling roots).

 

 

 

 

 

 

收藏該商鋪

請(qǐng) 登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~
二維碼 意見反饋
在線留言