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期刊：Journalof integrative plant biology（JIPB）

主題：OsCIPK7改變激酶活性調(diào)控水稻耐寒機(jī)制（鈣信號)

標(biāo)題：OsCIPK7point-mutation leads to conformation and kinase-activity change for sensingcold response

影響因子：3.129

檢測指標(biāo)：Ca2+

作者：中科院植物所 種康

英文摘要

Calcineurin B-like interacting proteinkinases (CIPKs) play important roles via environmental stress. However, less isknown how to sense the stress in molecular structure conformation level.

Here, an OsCIPK7 mutant via TILLINGprocedure with a point mutation in the kinase domain showed increased chillingtolerance, which could be potentially used in the molecular breeding.

We found that this point mutation ofOsCIPK7 led to a conformational change in the activation loop of the kinasedomain, subsequently with an increase of protein kinase activity, thusconferred an increased tolerance to chilling stress.

中文摘要（谷歌機(jī)翻）

鈣調(diào)神經(jīng)磷酸酶B樣相互作用蛋白激酶（CIPKs）通過環(huán)境壓力發(fā)揮重要作用。 然而，人們對如何感知分子結(jié)構(gòu)構(gòu)象水平的應(yīng)力知之甚少。

在此，通過TILLING程序在激酶結(jié)構(gòu)域中具有點(diǎn)突變的OsCIPK7突變體顯示出增加的耐寒性，其可能潛在地用于分子育種。

我們發(fā)現(xiàn)OsCIPK7的這一點(diǎn)突變導(dǎo)致激酶結(jié)構(gòu)域的激活環(huán)的構(gòu)象變化，隨后隨著蛋白激酶活性的增加，因此賦予對低溫脅迫的增加的耐受性。

NMT measurements show dynamic change of extracellular Ca2+ in?ux in live roots of various genetic backgrounds responding to cold shock. The blue background represents the duration of cold treatment. The number was the cold treatment time on the abscissa. The minus numbers were the time before the treatment.

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