山東農(nóng)大丨磁處理對(duì)鹽脅迫下楊樹(shù)氮吸收與分布的影響(附NMT實(shí)驗(yàn)體系)
期刊:Scientific Reports
主題:磁處理對(duì)鹽脅迫下楊樹(shù)氮吸收與分布的影響
標(biāo)題:The effects of magnetic treatment on nitrogen absorption and distribution in seedlings of Populus × euramericana ‘Neva’ under NaCl stress
影響因子:4.011
檢測(cè)指標(biāo):NH4+、NO3-流速
檢測(cè)部位:楊樹(shù)根部(伸長(zhǎng)區(qū)距離根尖15mm)葉肉細(xì)胞
NH4+、NO3-流速流實(shí)驗(yàn)處理方法:
楊樹(shù)幼苗,在0g/L NaCl+磁化(M0),0g/L NaCl+非磁化(NM0),4g/L NaCl+磁化(M4),4g/L NaCl+非磁化(NM4)中處理
NH4+、NO3-流速流實(shí)驗(yàn)測(cè)試液成份:
NH4+: 0.1 mM NH4NO3, 0.1 mM CaCl2, 0.3 mM MES, pH 5.5
NO3-: 0.1 mM NH4NO3, 1.0 mM KCl, 0.1 mM CaCl2, 0.3 mM MES, pH 5.5
作者:山東農(nóng)業(yè)大學(xué)林學(xué)院馬風(fēng)云、劉秀梅
英文摘要
A potted experiment with Populus × euramericana ‘Neva’ was carried out to assess whether there are positive effects of magnetic treatment of saline water (MTSW) on nitrogen metabolism under controlled conditions in a greenhouse.
Growth properties, nitrogen contents, enzyme activities and metabolite concentrations were determined based on field experiments and laboratory analysis after a 30-day treatment.
The results were as follows: (1) Biomass accumulation, root morphological properties and total nitrogen content were improved by MTSW. (2) Magnetization led to a greater increase in nitrate-nitrogen (NO3−-N) content in roots than in leaves, accompanied by greater NO3− efflux and activated nitrate reductase. (3) MTSW led to a higher ammonium-nitrogen (NH4+-N) content and greater uptake of net NH4+ in the leaves than that in the roots. (4) Magnetization stimulated glutamine synthase, glutamate dehydrogenase and glutamate synthase activities, whereas the concentrations of glutathione and oxidized glutathione were increased in leaves but decreased in roots, and the total glutathione content was increased.
Overall, these results indicated some beneficial impacts of MTSW on nitrogen translocation under field conditions, especially for equilibrating the distribution of NO3−-N and NH4+-N. Moreover, these findings confirmed the potential of using low-quality water for agriculture.
中文摘要
進(jìn)行了歐洲楊(Populus×euramericana'Neva')的盆栽試驗(yàn),以評(píng)估在溫室中受控條件下鹽水(MTSW)磁處理對(duì)氮代謝的積極影響。
在30天處理后,基于田間實(shí)驗(yàn)和實(shí)驗(yàn)室分析確定生長(zhǎng)特性,氮含量,酶活性和代謝物濃度。
結(jié)果如下:(1)MTSW提高了生物量積累,根系形態(tài)特征和總氮含量。(2)磁化導(dǎo)致根中硝態(tài)氮(NO3-N)含量比葉片更大,伴隨著更多的NO3-外排和活化的硝酸還原酶。(3)MTSW導(dǎo)致葉片中銨態(tài)氮(NH4+-N)含量較高,凈NH4+吸收量高于根系。 (4)磁化刺激谷氨酰胺合成酶,谷氨酸脫氫酶和谷氨酸合酶活性,而谷胱甘肽和氧化型谷胱甘肽的濃度在葉片中增加但在根中減少,總谷胱甘肽含量增加。
總體而言,這些結(jié)果表明MTSW對(duì)田間條件下氮轉(zhuǎn)運(yùn)的一些有益影響,特別是對(duì)于平衡NO3- N和NH4+-N的分布。此外,這些發(fā)現(xiàn)證實(shí)了使用劣質(zhì)水進(jìn)行農(nóng)業(yè)的潛力。
結(jié)果表明:使用NMT技術(shù)監(jiān)測(cè)葉肉細(xì)胞中和距離根尖15mm的伸長(zhǎng)區(qū)NO3-凈流速,兩者均顯示外排。在測(cè)量的溶液中,葉肉細(xì)胞的NO3-外排從235.09增加到290.23 pmol cm-2 s-1(圖A),暴露于NaCl溶液中,細(xì)根中從123.53到157.51 pmol cm-2 s-1顯著增加(p <0.05;圖B)。在暴露于鹽脅迫的植物中這些值比在對(duì)照中更高。當(dāng)暴露于鹽水溶液時(shí),葉片和根部的NO3-流速增加,并且葉肉細(xì)胞中的外排量大于根伸長(zhǎng)區(qū)域中的外排量。與非磁處理組中的流速相比,NO3-流速在暴露于磁場(chǎng)條件下的葉肉細(xì)胞中顯示出顯著更高的速率。吸收大的為M4(290.23 pmol cm-2 s-1),低吸收量為NM0(181.42 pmol cm-2 s-1)。然而,NO3-流速在根中表現(xiàn)出相反的模式,在伸長(zhǎng)區(qū)域中的外排顯著降低;NO3-外排為NM4(157.51 pmol cm-2 s-1),外排量為M0(38.05 pmol cm-2 s-1)
結(jié)果表明:NH4+的凈流速不同于NO3-;葉肉細(xì)胞為吸收趨勢(shì)(圖A),根部為外排趨勢(shì)(圖B)。葉中NH4+的吸收顯示出較低的值。暴露于NaCl溶液(M4,NM4)的樣品和暴露于對(duì)照處理(M0,NM0)的樣品相比,M0顯示出大的流入量(-2518.85pmol cm-2 s-1),M4顯示出第二大流出量(1164.15pmol cm-2 s-1),他們有著顯著不同(p<0.05)。 此外,NM4中NH4+通量的值與NM0非常接近,并且它們彼此沒(méi)有差異。相反,鹽脅迫下新生長(zhǎng)的細(xì)根中NH4+的外排高于對(duì)照中觀察到的,并且M4顯示出的外排量(186.83 pmolcm-2 s-1),與M0相比顯著不同(p <0.05)。如在葉中觀察到的,NM4中的NH4+外排與根中的NM0相似,并且數(shù)值沒(méi)有差異。與鹽水處理的效果不同,磁處理誘導(dǎo)NH4+的外排比非磁處理更大,并且M4和M0都顯示出比NM4和NM0更高的值。這兩個(gè)因素之間也存在顯著的相互作用(p <0.05)