江蘇師范大學(xué)多倍體維持鈉鉀穩(wěn)態(tài)促耐鹽能力的新機(jī)制
NMT是基因功能的活體檢測技術(shù),已被31位諾貝爾獎得主所在單位,及北大、清華、中科院使用。
2019年10月江蘇師大NMT合作研究中心(測試中心)服務(wù)信息
期刊:Journal of Experimental botany
主題:鉀離子和鈣離子可滲透通道對H2O2的根區(qū)特異性敏感性決定了鹽堿化二倍體和六倍體三叉戟的離子xxxxx
標(biāo)題:Root-zone-specific sensitivity of K+-and Ca2+-permeable channels to H2O2 determines ion homeostasis in salinized diploid and hexaploid Ipomoea trifida
影響因子:5.354
檢測指標(biāo):K+、H+、Ca2+、Na+流速
檢測部位:甘薯
K+、H+、Ca2+瞬時(shí):分生區(qū)(距離根尖500μm處),伸長(距3毫米),成熟(15毫米)
K+、Na++:分生組織區(qū)(距離300-600μm)測量間隔為100μm),伸長區(qū)(1-3 mm)測量間隔為500μm的和成熟區(qū)(距10-15毫米,測量間隔為1毫米)
K+、H+、Ca2+、Na+流速流實(shí)驗(yàn)處理方法:
甘薯幼苗,150mM NaCl處理7d/150mM NaCl瞬時(shí)處理
K+、H+、Ca2+、Na+流速流實(shí)驗(yàn)測試液成份:
H+/K+/Ca2+瞬時(shí):0.1 mM NaCl, 0.1 mM MgCl2,0.1 mM CaCl2, and 0.5 mM KCl at pH 5.7
K+:150 mM NaCl, 0.1 mM MgCl2, 0.1 mM CaCl2, and 0.5 mM KCl, pH 5.7
作者:江蘇師范大學(xué)孫健、李宗蕓
英文摘要
Polyploids generally possess superior K+/Na+ homeostasis under saline conditions compared with their diploid progenitors.
In this study, we identified the physiological mechanisms involved in the ploidy-related mediation of K+/Na+ homeostasis in the roots of diploid (2x) and hexaploid (6x; autohexaploid) Ipomoea trifida, which is the closest relative of c*ted sweet potato. Results showed that 6x I. trifida retained more K+ and accumulated less Na+ in the root and leaf tissues under salt stress than 2x I. trifida.
Compared with its 2x ancestor, 6x I. trifida efficiently prevents K+ efflux from the meristem root zone under salt stress through its plasma membrane (PM) K+-permeable channels, which have low sensitivity to H2O2. Moreover, 6x I. trifida efficiently excludes Na+ from the elongation and mature root zones under salt stress because of the high sensitivity of PM Ca2+-permeable channels to H2O2.
Our results suggest the root-zone-specific sensitivity to H2O2 of PM K+- and Ca2+-permeable channels in the co-ordinated control of K+/Na+ homeostasis in salinized 2x and 6x I. trifida. This work provides new insights into the improved maintenance of K+/Na+ homeostasis of polyploids under salt stress.
中文摘要(谷歌機(jī)翻)
與二倍體祖細(xì)胞相比,多倍體在鹽水條件下通常具有*的K+ / Na+穩(wěn)態(tài)。
在這項(xiàng)研究中,我們確定了與二倍體(2x)和六倍體(6x;自六倍體)Tripoda的根的K+/ Na+穩(wěn)態(tài)的倍性相關(guān)介導(dǎo)有關(guān)的生理機(jī)制,這是栽培紅薯的近親。結(jié)果表明,與2x I. trifida相比,在鹽脅迫下6x I. trifida保留更多的K +并在根和葉組織中積累較少的Na+。
與2x祖先相比,6x裂葉線蟲通過其對H2O2敏感性低的質(zhì)膜(PM)K+滲透通道有效地防止了鹽脅迫下分生組織根區(qū)的K+流出。此外,由于PM Ca2+的可滲透通道對H2O2的敏感性高,因此6x I. trifida有效地將Na+從鹽脅迫下的伸長和成熟根區(qū)中排除。
我們的結(jié)果表明,在鹽漬化2x和6x鹽粉虱的K+ / Na+動態(tài)平衡控制中,PM K+-和Ca2+滲透通道對H2O2的根區(qū)特異性敏感性。這項(xiàng)工作為改善鹽脅迫下多倍體K+ / Na+穩(wěn)態(tài)的維持提供了新的見解。
結(jié)果表明:在分生組織區(qū)中,觀察到更高的K+流出量的是2x,在鹽處理(約30分鐘)期間,鹽誘導(dǎo)的K+滲漏的平均速率高達(dá)4800 pmol cm-2 s-1(比6x高1.9倍)。但是,在伸長和成熟的根部區(qū)域觀察到相反的趨勢,其中6x比2x表現(xiàn)出更強(qiáng)烈的K+外排。