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NMT是基因功能的活體檢測(cè)技術(shù)，已被31位諾貝爾獎(jiǎng)得主所在單位，及北大、清華、中科院使用。?

NMT歷*的今天

2016年11月13日，中科院煙臺(tái)海岸帶研究所駱永明、李連禎利用NMT在Plant and Soil 上發(fā)表了標(biāo)題為Determining the fluxes of ions (Pb2+, Cu2+ and Cd2+) at the root surface of wetland plants using the scanning ion-selective electrode technique的研究成果。

期刊：Plant and Soil

主題：使用掃描離子選擇電極技術(shù)確定濕地植物根表面離子（Pb2+，Cu2+和Cd2+）的通量

標(biāo)題：Determining the fluxes of ions (Pb2+, Cu2+ and Cd2+) at the root surface of wetland plants using the scanning ion-selective electrode technique

影響因子：3.410

檢測(cè)指標(biāo)：Pb2+、Cu2+、Cd2+流速

作者：中科院煙臺(tái)海岸帶研究所駱永明、李連禎

英文摘要

Measuring specific ion fluxes from different regions of the root under practical physiological conditions is crucial for understanding metal uptake mechanisms by plants.

We developed and tested a neutral carrier-based liquid-membrane Pb2+ and Cu2+ ion selective microelectrode (ISME) to investigate ion-transport processes along the roots of three common wetland plant species.

The Pb2+ and Cu2+ ISME exhibited a Nernstian response with Pb2+ and Cu2+ activities as low as 1.0 nM and 1.0 μM in deionized water and simulated soil solution, respectively. Phragmites australis had a region of Cu2+ release for approximately the first 200 μm, while it exhibited Pb2+ and Cd2+ outward net flux up to the first 500 μm. Although in older sections of the root of Phragmites australis there were areas of influx of Cu2+, Pb2+ and Cd2+, the overall influx was much smaller than that of Typha latifolia or Canna indica. Such a reduced uptake and/or an increased efflux of metal ions across the root-cell plasma-membrane might explain the higher resistance of Phragmites australis to metals, at least in part.

The Pb2+ and Cu2+ ISMEs are shown to permit detailed investigation of heavy-metal ion transport in plant roots, especially for plants used for phytoremediation.

中文摘要（谷歌機(jī)翻）

在實(shí)際的生理?xiàng)l件下，測(cè)量來(lái)自根不同區(qū)域的比離子通量對(duì)于理解植物對(duì)金屬的吸收機(jī)理至關(guān)重要。

我們開(kāi)發(fā)并測(cè)試了基于中性載體的液膜Pb2+和Cu2+離子選擇性微電極（ISME），以研究沿三種常見(jiàn)濕地植物物種根部的離子遷移過(guò)程。

在去離子水和模擬土壤溶液中，Pb2+和Cu2+的ISME表現(xiàn)出Nernstian響應(yīng)，Pb2+和Cu2+的活性分別低至1.0 nM和1.0μM。蘆葦在大約初的200μm處具有Cu2+釋放區(qū)域，而在初的500μm處表現(xiàn)出Pb2+和Cd2+向外凈通量。盡管在蘆葦根部的較老部分中有Cu2+，Pb2+和Cd2+的流入?yún)^(qū)域，但總流入量比香蒲或印度香豆小得多。穿過(guò)根細(xì)胞質(zhì)膜的這種減少的金屬離子吸收和/或增加的金屬離子外流可能至少部分解釋了蘆葦對(duì)金屬的更高抗性。

研究表明，Pb2+和Cu2+ ISME可以詳細(xì)研究植物根特別是用于植物修復(fù)的植物中重金屬離子的轉(zhuǎn)運(yùn)。

Fig. 3 Measurement of fluxes (outward positive) of Cd2+,Pb2+ and Cu2+ (mean ± standard error) across the root tips of three common wetland plant species, Typha latifolia (a) Canna indic (b) and Phragmites australis (c), using Cd2+,Pb2+ and Cu2+ ion selective microelectrodes and the scanning ion-selective electrode higher metal influx in the root regions, especially in the meristematic and elongation zone (Fig. 3).