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NMT歷*的今天丨中農(nóng)學(xué)者：非損傷NMT設(shè)備檢測(cè)K+流的穩(wěn)定性
??NMT作為生命科學(xué)底層核心技術(shù)，是建立活體創(chuàng)新科研平臺(tái)的*技術(shù)。2005年~2020年，NMT已扎根中國(guó)15年。2020年，中國(guó)NMT銷往瑞士蘇黎世大學(xué)，正式打開(kāi)歐洲市場(chǎng)
NMT歷*的今天
2016年05月13日，中國(guó)農(nóng)業(yè)大學(xué)王忠義用NMT發(fā)表了標(biāo)題為The calibration model in potassium ion flux non-invasive measurement of plants in vivo in situ的研究成果。
期刊：Information Processing in Agriculture
主題：非損傷NMT設(shè)備檢測(cè)K+流的穩(wěn)定性
標(biāo)題：The calibration model in potassium ion flux non-invasive measurement of plants in vivo in situ
檢測(cè)指標(biāo)：K+流速
作者：中國(guó)農(nóng)業(yè)大學(xué)王忠義
英文摘要
NMT (Non-invasive Micro-test Technology) provides a novel electrophysiological tool which can non-invasively measure the dynamic influxes and effluxes of ions caused by the diffusion along the concentration gradients in vivo.
However, in this technique ion fluxes are converted to voltage signals using an ion selective microelectrode at a small amplitude of μV, which is easy to be interfered by the ambient noise. Hence, effective solutions to the suppression of noise and calibration of ion flux measurement system are very important for this method. A K+-selective microelectrode was constructed using liquid ion exchangers (LIX) to investigate ion transport over plant tissue.
A standard concentration gradient which simulates plant living cells was produced by an electrode with a certain tip diameter, filled with a solution containing a known K+ concentration in 100 mmol/L. An ion diffusion simulation model was established. This model evaluated the performance of ion flux measurement system in accuracy and reliability by comparing the consistency of the measured value and the predicted curve. K+ fluxes were measured within 25 minutes at each measuring point of distance 10, 20, 30, 40, 50, 80, and 100 μm from the K+ source, respectively.
It can be seen that the K+ fluxes changes little, which indicates that ion fluxes measurement system has a reliable stability. The study provides a theoretical basis for a new non-invasive ion flux measurement method creation and a new sensors design.
中文摘要（谷歌機(jī)翻）
NMT（非損傷微測(cè)技術(shù)）提供了一種新型的電生理工具，可以無(wú)創(chuàng)地測(cè)量由于體內(nèi)濃度梯度的擴(kuò)散所引起的離子的動(dòng)態(tài)流入和流出。
然而，在該技術(shù)中，使用離子選擇微電極以小幅度的μV將離子通量轉(zhuǎn)換為電壓信號(hào)，這容易受到環(huán)境噪聲的干擾。因此，有效的解決方案包括抑制噪聲和校準(zhǔn)離子通量測(cè)量系統(tǒng)。使用液體離子交換劑（LIX）構(gòu)建K+選擇性微電極，以研究離子在植物組織上的傳輸。
由具有一定直徑的電極產(chǎn)生的模擬植物活細(xì)胞的標(biāo)準(zhǔn)濃度梯度，該電極填充有含有100 mmol / L已知K+濃度的溶液。建立了離子擴(kuò)散模擬模型。該模型通過(guò)比較測(cè)量值和預(yù)測(cè)曲線的一致性來(lái)評(píng)估離子通量測(cè)量系統(tǒng)在準(zhǔn)確性和可靠性方面的性能。在25分鐘內(nèi)分別在距K +源10、20、30、40、50、80和100μm的每個(gè)測(cè)量點(diǎn)測(cè)量K+通量。
可以看出，K+通量變化很小，表明離子通量測(cè)量系統(tǒng)具有可靠的穩(wěn)定性。該研究為新的非侵入式離子通量測(cè)量方法的創(chuàng)建和新的傳感器設(shè)計(jì)提供了理論基礎(chǔ)。
Fig. 8. K+ flux measured at each known position (vibration amplitude Δx is 10