自然 通訊:質(zhì)子流在?植物低磷適應(yīng)研究上的作用|NMT磷營養(yǎng)創(chuàng)新平臺
NMT作為生命科學(xué)底層核心技術(shù),是建立活體創(chuàng)新科研平臺的*技術(shù)。2005年~2020年,NMT已扎根中國15年。2020年,中國NMT銷往瑞士蘇黎世大學(xué),正式打開歐洲市場。
研究使用平臺:NMT磷營養(yǎng)創(chuàng)新科研平臺
期刊:Nature Communications
主題:質(zhì)子流在白羽扇豆基因組進化和低磷適應(yīng)研究上的關(guān)鍵作用
標(biāo)題:The genome evolution and low-phosphorus adaptation in white lupin
影響因子:11.878
檢測指標(biāo):H+流速
檢測樣品:白羽扇豆
H+流實驗處理方法:
檢測簇狀根和正常根
H+流實驗測試液成份:
0.1mM KCl,0.1mM CaCl2、0.1mM MgCl2、0.5mM NaCl,0.3mM MES,0.2mM Na2SO4,pH 6.0
作者:福建農(nóng)林大學(xué)許衛(wèi)鋒、張仟、夏天雨;中國農(nóng)業(yè)科學(xué)院蔬菜花卉研究所程鋒
中文摘要(谷歌機翻)
白羽扇豆(羽扇豆(Lupinus albus))是一種豆科植物,可生長簇狀根,在低磷土壤中具有較高的磷(P)利用效率(PUE)。
在這里,我們組裝了白羽扇豆的基因組,發(fā)現(xiàn)它是從全基因組三倍(WGT)事件演變而來的。然后,我們破譯其二倍體祖先基因組并重建三個亞基因組。
根據(jù)結(jié)果,我們進一步揭示了不同亞基因組的亞基因組優(yōu)勢和基因表達,這些基因組的表達隨其轉(zhuǎn)座子(TE)密度而變化。白羽扇豆中的PUE基因已通過WGT以及串聯(lián)和分散重復(fù)進行了擴增。
此外,我們表征了高PUE的四個主要途徑,包括碳固定,簇根形成,土壤P固定和細胞P重用。其中,生長素調(diào)節(jié)可能通過參與LaABCG37s對簇根形成很重要。這些發(fā)現(xiàn)為白羽扇豆的基因組進化和低磷適應(yīng)提供了見識。
( b ) Hydrolytic activity and H+ flux rate of PM H+-ATPase in different types of roots. n = 3 plants for PM ATPaseactivity and 4 plants for H+ flux . ( c ) Effects of vanadate ( Na3VO4 , inhibitor of P-type ATPase ) on H+ flux rate and cluster root formation in white lupin under P sufficient and deficient condition. n = 4 plants . Error bars indicate s.e. m., P value was calculated using the unpaired two-sided Student's t-test . + PR , P sufficient roots ; - PNR , P deficientmomal roots ; -PCR P deficient cluster roots ; Lane 1 . + PR ; Lane 2 , - PNR ; Lane 3,PCR . Source data are provided as a Source Data file.
英文摘要
White lupin (Lupinus albus) is a legume crop that develops cluster roots and has high phosphorus (P)-use efficiency (PUE) in low-P soils.
Here, we assemble the genome of white lupin and find that it has evolved from a whole-genome triplication (WGT) event. We then decipher its diploid ancestral genome and reconstruct the three sub genomes.
Based on the results, we further reveal the sub-genome dominance and the genic expression of the different sub-genomes varying in relation to their transposable element (TE) density. The PUE genes in white lupin have been expanded through WGT as well as tandem and dispersed duplications.
Furthermore, we characterize four main pathways for high PUE, which include carbon fixation, cluster root formation, soil-P remobilization, and cellular-P reuse. Among these, auxin modulation may be important for cluster root formation through involvement of LaABCG37s. These findings provide insights into the genome evolution and low-P adaptation of white lupin.
關(guān)鍵詞:非損傷微測技術(shù),H+流速,白羽扇豆