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NMT歷*的今天丨棉花纖維Ca2+流研究
NMT作為生命科學(xué)底層核心技術(shù)，是建立活體創(chuàng)新科研平臺的*技術(shù)。2005年~2020年，NMT已扎根中國15年。2020年，中國NMT銷往瑞士蘇黎世大學(xué)，正式打開歐洲市場
NMT歷*的今天
2014年05月26日，華中農(nóng)業(yè)大學(xué)涂禮莉用NMT發(fā)表了標(biāo)題為Down-regulating annexin gene GhAnn2 inhibits cotton fiber elongation and decreases Ca2+ influx at the cell apex的研究成果。
期刊：Plant Molecular Biology
主題：下調(diào)膜聯(lián)蛋白基因GhAnn2抑制棉纖維伸長并減少細(xì)胞的Ca2+流入
標(biāo)題：Down?regulating annexin gene GhAnn2 inhibits cotton fiber elongation and decreases Ca2+ influx at the cell apex
檢測指標(biāo)：Ca2+流速
作者：華中農(nóng)業(yè)大學(xué)涂禮莉、唐文鑫
英文摘要
Cotton fiber is a single cell that differentiates from the ovule epidermis and undergoes synchronous elongation with high secretion and growth rate. Apart from economic importance, cotton fiber provides an excellentMsingle-celled model for studying mechanisms of cellgrowth.
Annexins are Ca2+ and phospholipid-binding proteins that have been reported to be localized in multiple cellular compartments and involved in control of vesicle secretions. Although several annexins have been found to be highly expressed in cotton fibers, their functional roles in fiber development remain unknown.
Here, 14 annexin family members were identified from the fully sequenced diploid G. raimondii (D5 genome), half of which were expressed in fibers of the c*ted tetraploid species G. hirsutum (cv. YZ1). Among them, GhAnn2 from the D genome of the tetraploid species displayed high expression level in elongating fiber. The expression of GhAnn2 could be induced by some phytohormones that play important roles in fiber elongation, such as IAA and GA3. RNAimediated down-regulation of GhAnn2 inhibited fiber elongation and secondary cell wall synthesis, resulting in shorter and thinner mature fibers in the transgenic plants.
Measurement with Non-invasive Micro-test Technology revealed that the rate of Ca2+ influx from extracellular to intracellular was decreased at the fiber cell apex of GhAnn2 silencing lines, in comparison to that in the wild type. These results indicate that GhAnn2 may regulate fiber development through modulating Ca2+ fluxes and signaling.
中文摘要（谷歌機(jī)翻）
棉纖維是與胚珠表皮不同的單個細(xì)胞，并以高分泌和高生長速率經(jīng)歷同步伸長。除了經(jīng)濟(jì)上的重要性外，棉纖維還為研究細(xì)胞生長機(jī)理提供了出色的單細(xì)胞模型。
膜聯(lián)蛋白是Ca2+和磷脂結(jié)合蛋白，據(jù)報道它們位于多個細(xì)胞區(qū)室并參與囊泡分泌的控制。盡管已經(jīng)發(fā)現(xiàn)幾種膜聯(lián)蛋白在棉纖維中高度表達(dá)，但是它們在纖維發(fā)育中的功能作用仍然未知。
在這里，從*測序的二倍體雷蒙德氏酵母（D5基因組）中鑒定出14個膜聯(lián)蛋白家族成員，其中一半在培養(yǎng)的四倍體物種hirsutum（cv。YZ1）的纖維中表達(dá)。其中，來自四倍體物種的D基因組的GhAnn2在伸長纖維中顯示高表達(dá)水平。GhAnn2的表達(dá)可以被一些在纖維伸長中起重要作用的植物激素誘導(dǎo)，例如IAA和GA3。RNAi介導(dǎo)的GhAnn2的下調(diào)抑制了纖維的伸長和次級細(xì)胞壁的合成，從而導(dǎo)致轉(zhuǎn)基因植物中成熟纖維的長度越來越短。
通過非損傷微測技術(shù)進(jìn)行的測量顯示，與野生型相比，GhAnn2沉默系的纖維細(xì)胞頂點從細(xì)胞外到細(xì)胞內(nèi)的Ca2+流入速率降低。這些結(jié)果表明，GhAnn2可能通過調(diào)節(jié)Ca2+通量和信號傳導(dǎo)來調(diào)節(jié)纖維發(fā)育。
Fig. 4 RNAi silencing of GhAnn2 decreases the rate of Ca2+ influx at the fiber cell apex. a Tip Ca2+ flux of 5 DPA fiber cells was measured by noninvasive scanning ion-selective electrode. Four bolls in one line were analyzed, and at least three fiber cells in one boll were tested. b Average rate of Ca2+ flux of 5 DPA fiber cells. Error bars indicate the SD of three biological replicates.