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NMT作為生命科學底層核心技術，是建立活體創(chuàng)新科研平臺的*技術。2005年~2020年，NMT已扎根中國15年。2020年，中國NMT銷往瑞士蘇黎世大學，正式打開歐洲市場。

NMT歷*的今天

2018年07月01日，廣東省科學院生態(tài)環(huán)境研究所李芳柏用NMT發(fā)表了標題為Selenium reduces cadmium uptake into rice suspension cells by regulating the expression of lignin synthesis and cadmium-related genes的研究成果。

 期刊：Science of The Total Environment

主題：硒通過調節(jié)木質素合成和鎘相關基因的表達減少水稻對懸浮細胞的鎘吸收
標題：Selenium reduces cadmium uptake into rice suspension cells by regulating the expression of lignin synthesis and cadmium-related genes

檢測指標：Cd²⁺流速

作者：廣東省科學院生態(tài)環(huán)境研究所李芳柏、崔江虎

英文摘要

Although previous studies have indicated that selenium (Se) can reduce cadmium (Cd) uptake into rice, the mechanism at the cellular level has not been reported. Here, rice suspension cells exposed to Cd treatment in the presence or absence of Se were  Compared with treatment with alone, pretreatment with Se increased the proportion of live cells by 83.1%. The levels of reactive oxygen species and mitochondrial membrane potential in the Se-pretreated rice cells were decreased by 86.6% and 76.0%, respectively.

In addition, non-invasive micro-test technology suggested that the mean values of Cd²⁺ influx decreased significantly in the Se-pretreated rice cells in a concentration-dependent manner. The results of inductively coupled plasmamass spectrometry (ICP-MS) showed that 67.4%–78.8% Cd accumulated onto the cell walls of the pretreated-Se rice cells. The addition of Se increased the lignin content and thickness of the cell walls, leading to an improved mechanical force of the cell walls, as determined by atomic force microscopy (AFM).

Furthermore, Se pretreatment decreased the expression of genes involved in Cd uptake (OsNramp5) and transport (OsLCT1) but activated the expression of genes involved in Cd transport into vacuoles (OsHMA3) and lignin synthesis (OsPAL, OsCoMT and Os4CL3). These results indicated that supplying Se alleviates Cd toxicity by regulating the express of lignin synthesis and Cd-related genes. The present findings provide new insights on a plausible explanation of the Se-reduced Cd uptake into rice.

中文摘要

盡管以前的研究表明硒可以減少水稻對鎘的吸收，但是在細胞水平上的機制尚未見報道。在此，將在有硒或無硒的情況下暴露于Cd處理的水稻懸浮細胞與單獨處理相比，用硒進行預處理可使活細胞的比例增加83.1％。硒預處理的水稻細胞中的活性氧水平和線粒體膜電位分別降低了86.6％和76.0％。

此外，非損傷微測技術表明，硒預處理的水稻細胞中Cd²⁺流入量的平均值以濃度依賴的方式顯著降低。電感耦合等離子體質譜法（ICP-MS）的結果表明，預處理的Se水稻細胞的細胞壁上積累了67.4％–78.8％的Cd。Se的添加增加了木質素的含量和細胞壁的厚度，從而導致了細胞壁機械力的改善，這是通過原子力顯微鏡（AFM）確定的。

此外，Se預處理降低了Cd吸收（OsNramp5）和轉運（OsLCT1）相關基因的表達，但激活了Cd轉運至液泡（OsHMA3）和木質素合成（OsPAL，OsCoMT和Os4CL3）的基因表達。這些結果表明提供硒通過調節(jié)木質素合成和Cd相關基因的表達來減輕Cd毒性。目前的發(fā)現(xiàn)為對水稻中硒減少的鎘吸收的合理解釋提供了新的見解。

 (A) Representative cell and the Cd²⁺-selective microelectrode; (B) The average Cd²⁺ flux values (mean ± SD) in the rice cells was detected in the absence or presence of Se.