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NMT揭示楊樹不同根區(qū)NO3-吸收差異與miRNA調(diào)節(jié)有關(guān)
NMT作為生命科學(xué)底層核心技術(shù)，是建立活體創(chuàng)新科研平臺的*技術(shù)。2005年~2020年，NMT已扎根中國15年。2020年，中國NMT銷往瑞士蘇黎世大學(xué)，正式打開歐洲市場。
研究使用平臺：NMT植物營養(yǎng)創(chuàng)新科研平臺
期刊：Tree Physiology
主題：NMT揭示楊樹不同根區(qū)NO3-吸收差異與miRNA調(diào)節(jié)有關(guān)
標(biāo)題：Physiological characteristics and RNA sequencing in two root zones with contrasting nitrate assimilation of Populus × canescen
影響因子：3.655
檢測指標(biāo)：NO3-流速
檢測樣品：灰楊根部
NO3-流實(shí)驗(yàn)處理方法：0.5 mM NaNO3 培養(yǎng)10d
NO3-流實(shí)驗(yàn)測試液成份：0.5 mM NaNO3，pH 5.8
作者：中國林業(yè)科學(xué)研究院林業(yè)研究所羅志斌、周婧
中文摘要
不同的根區(qū)在胡楊屬物種中具有不同的吸收硝酸鹽（NO3-）的能力，但基本的生理和微RNA（miRNA）調(diào)節(jié)機(jī)制仍然未知。
為了解決這個問題，研究了兩個具有不同NO3吸收能力的根莖假單胞菌的根區(qū)。到根尖的0至40 mm區(qū)域（根部區(qū)域I）顯示出凈流入，而40至80 mm的區(qū)域（根部區(qū)域II）顯示出凈流出。II區(qū)的NO3-和銨（NH4+）濃度以及硝酸還原酶（NR）活性低于I區(qū)。
與區(qū)域I相比，在區(qū)域II中鑒定了41個上調(diào)的miRNA和23個下調(diào)的miRNA，以及這些miRNA的576個靶標(biāo)。尤其是生長調(diào)節(jié)因子4（GRF4），即ptc-miR396g-5p和ptc-miR396f_L上調(diào)的靶標(biāo)與區(qū)域I相比，區(qū)域II中的+ 1R-1被下調(diào)，可能有助于區(qū)域II中較低的NO3吸收率和吸收。
此外，在根區(qū)發(fā)現(xiàn)了一些miRNA及其靶標(biāo)，即C2H2鋅指家族成員和APETALA2 /乙烯反應(yīng)性元素結(jié)合蛋白家族成員，這可能在調(diào)節(jié)NO3-吸收中起重要作用。這些結(jié)果表明，差異表達(dá)的miRNA-靶標(biāo)對在調(diào)節(jié)不同NO3-吸收速率以及在楊樹不同根部區(qū)域的同化中起關(guān)鍵作用。
英文摘要
Different root zones have distinct capacities for nitrate (NO3-) uptake in Populus species, but the underlying physiological and microRNA (miRNA) regulatory mechanisms remain largely unknown.
To address this question, two root zones of P. × canescens with contrasting capacities forNO3- uptake were investigated. The region of 0 to 40 mm (root zone I) to the root apex displayed net influxes, whereas the region of 40 to 80 mm (root zone II) exhibited net effluxes. Concentrations of NO3- and ammonium (NH4+) as well as nitrate reductase (NR) activity were lower in zone II than in zone I.
Forty one upregulated and twenty three downregulated miRNAs, and 576 targets of these miRNAs were identified in zone II in comparison with zone I. Particularly, growth-regulating factor 4 (GRF4), a target of upregulated ptc-miR396g-5p and ptc-miR396f_L + 1R-1, was downregulated in zone II in comparison with zone I, probably contributing to lower NO3-uptake rates and assimilation in zone II.
Furthermore, several miRNAs and their targets, members of C2H2 zinc finger family and APETALA2/ethylene-responsive element binding protein family, were found in root zones, which probably play important roles in regulating NO3- uptake. These results indicate that differentially expressed miRNA-target pairs play key roles in regulation of distinct NO3-uptake rates and assimilation in different root zones of poplars.