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DNase I
（Deoxyribonuclease I）, can digest single or double-stranded DNA to produce mono
deoxynucleotides or single or double-stranded oligo deoxynucleotides, its
optimal working pH range is 7-8. DNase I activity is dependent on Ca2+
and can be activated by other bivalent metal ions such as Mg2+, Mn2+,
Zn2+, etc. In the presence of Mg2+, the enzyme can
randomly recognize and cut any site on any strand of DNA. In the presence of Mn2+,
two strands of DNA can be cut at the same site to form sticky ends with flat
ends or 1-2 nucleotides protruding.