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Endo-S2 is an endo-n-acetylglucosaminase derived from streptococcus pyogenes that excises the glycosidic bond between the two innermost n-acetylglucosamines of the glycoprotein n-linking sugar chain. It is effective for high mannose type, hybrid type and double antenna complex type. Can be made for all human IgG, as well as mice, rats, monkey, goat, sheep, cattle and horse IgG antibody glycosyl.