中文摘要：

植物的先天免疫限制了對全球食品安全構成威脅的細菌病原體的生長。然而，植物免疫抑制細菌生長的機制仍然不清楚。在這里，我們展示了擬南芥分泌的天冬氨酸蛋白酶1和2（SAP1和SAP2）切割進化上保守的細菌蛋白MucD，以重復地抑制細菌病原體銅綠假單胞菌的生長。SAP1的抗菌活性在植物內和體外都需要其蛋白酶活性。過表達SAP1的植物表現出增強的MucD切割和抗性，但在生長和繁殖上沒有受到影響，而sap1 sap2雙突變植物表現出MucD切割和對P. syringae的抗性受損。缺乏mucD的P. syringae在植物內和體外的生長受損。值得注意的是，在植物內和體外，缺乏可切割的MucDF106Y的ΔmucD的生長不受SAP活性的影響。我們的研究發(fā)現了植物抗菌機制背后的遺傳因素和生化過程。

英文摘要：

Plant innate immunity restricts growth of bacterial pathogens that threaten global food security. However, the mechanisms by which plant immunity suppresses bacterial growth remain enigmatic. Here we show that Arabidopsis thaliana secreted aspartic protease 1 and 2 (SAP1 and SAP2) cleave the evolutionarily conserved bacterial protein MucD to redundantly inhibit the growth of the bacterial pathogen Pseudomonas syringae. Antibacterial activity of SAP1 requires its protease activity in planta and in vitro. Plants overexpressing SAP1 exhibit enhanced MucD cleavage and resistance but incur no penalties in growth and reproduction, while sap1 sap2 double mutant plants exhibit compromised MucD cleavage and resistance against P. syringae. P. syringae lacking mucD shows compromised growth in planta and in vitro. Notably, growth of ΔmucD complemented with the non-cleavable MucDF106Y is not affected by SAP activity in planta and in vitro. Our findings identify the genetic factors and biochemical process underlying an antibacterial mechanism in plants.

論文信息：

論文題目：Site-specific cleavage of bacterial MucD by secreted proteases mediates antibacterial resistance in Arabidopsis

期刊名稱：Nature Communications

時間期卷：10, Article number: 2853 (2019)

在線時間：2019年6月28日

DOI：doi.org/10.1038/s41467-019-10793-x

產品信息：

貨號：101-A

規(guī)格：100ug

品牌：Virogen

產地：美國

名稱：Anti-Glutathione antibody

授權代理商：Target Technology（靶點科技）

檢測方式：WB

谷胱甘肽（glutathione，r-glutamyl cysteingl +glycine，GSH）是一種含γ-酰胺鍵和巰基的三肽，由谷氨酸、半胱氨酸及甘氨酸組成，存在于身體的幾乎每一個細胞。谷胱甘肽有還原型（G-SH）和氧化型（G-S-S-G）兩種形式，在生理條件下以還原型谷胱甘肽占絕大多數。谷胱甘肽還原酶可以催化兩型間的互變，該酶的輔酶還可以為磷酸戊糖旁路代謝提供的NADPH 。Virogen的抗谷胱甘肽抗體Anti-Glutathione antibody貨號101-A見刊于Nature Communications：

Liposoma巨噬細胞清除劑Clodronate Liposomes氯膦酸二鈉脂質體的材料和方法：

Virogen抗谷胱甘肽抗體Anti-Glutathione antibody貨號101-A專業(yè)論文WB數據參考