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CYT-439

重組小鼠巨噬細胞克隆刺激因子（M-CSF）

2μg/10μg/1mg

￥900/2280

Prospec

Background:
	Macrophage colony stimulating factor （M-CSF） is one of the glycoproteins called colony-stimulating factors （CSFs）.A mononuclear phagocyte colony-stimulating factor （M-CSF） synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo
	Description :
	Recombinant Mouse M-CSF produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4 KD. Recombinant M-CSF is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	The lyophilized protein （1mg/ml） was lyophilized with 5mM Tris, pH 8.0 + 100mM NaCl.
	Solubility:
	It is recommended to reconstitute the lyophilized Mouse M-CSF in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized Recombinant M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution M-CSF should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE silver-stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Glu-Val-Ser.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel.
	Biological Activity:
	ProSpec’s Mouse M-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 1ng/ml, corresponding to a Specific Activity of 1 x 10⁶ IU/mg.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of Recombinant Murine M-CSF.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of M-CSF as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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