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    重 組人細(xì)胞毒性T淋巴細(xì)胞相關(guān)抗原-4rHuCTLA-4)
    • 重 組人細(xì)胞毒性T淋巴細(xì)胞相關(guān)抗原-4rHuCTLA-4)
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    貨物所在地:上海上海市

    地: 以色列

    更新時(shí)間:2024-12-23 21:00:07

    瀏覽次數(shù):129

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    CYT-366 重組人細(xì)胞毒性T淋巴細(xì)胞相關(guān)抗原-4rHuCTLA-4)  20μg/100μg/1mg ¥900/2280 Prospec

     

    Background:
      CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximay 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization.

     
    Description :
      Recombinant Human CTLA-4 produced in insect cells is a homodimeric, glycosylated, polypeptide chain containing a total molecular mass of 80 kDa. Each subunit (40kDa) is fused to a polypeptide linker to the Fc portion of Human IgG1. CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximay 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo.
    Large doses of this soluble protein also suppress responses to a second immunization.
    Recombinant Human CTLA-4 is purified by proprietary chromatographic techniques.

     
    Physical Appearance:
      Sterile Filtered White lyophilized (freeze-dried) powder.

     
    Formulation:
      Recombinant CTLA-4 was lyophilized with no additives.

     
    Solubility:
      It is recommended to reconstitute the lyophilized CTLA4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

     
    Stability:
     
      Lyophilized CTLA4 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution CTLA-4 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please avoid freeze-thaw cycles.

     
    Purity:
      Greater than 95.0% as determined by:
    (a) Analysis by RP-HPLC.
    (b) Anion-exchange FPLC.
    (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

     
    Amino acid sequence:
      The sequence of the amino acids was determined and was found to be: Lys-Ala-Met-His-Val.

     
    Dimers and aggregates:
      Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

     
    Biological Activity:
      ProSpec’s Recombinant CTLA-4 is fully biologically active when compared to standards.

     
    Endotoxin:
      Less than 0.1 ng/µg (IEU/µg) of CTLA-4.

     
    Protein content:
      Protein quantitation was carried out by two independent methods:

    1. UV spectroscopy at 280.
    2. Analysis by RP-HPLC, using a standard solution of Human CTLA-4 as a Reference Standard.

     
    Usage:
      Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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