欧美国产日韩在线免费观看-欧美日韩成人激情一区二区-欧美久久综合一区二区-亚洲av寂寞少妇久久

訂貨：，，

Y-PER (R) Yeast Protein Extraction Reagent 介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

Y-PER (R) Yeast Protein Extraction Reagent 現(xiàn)貨供應(yīng)

Y-PER (R) Yeast Protein Extraction Reagent 品牌介紹

美國(guó)Pierce公司(www.pierce。。ｃｏｍ)是蛋白質(zhì)化學(xué)、免疫學(xué)和層析等很多方面的和*。http://www.thermo。。ｃｏｍ.cn/Category828.html

主要產(chǎn)品包括：

1)化學(xué)發(fā)光體和比色底物

2)分析試劑

3)熒光標(biāo)記探針

4)酶聯(lián)免疫分析試劑盒等

Y-PER (R) Yeast Protein Extraction Reagent 僅用于科研

The Thermo Scientific Y-PER Yeast Protein Extraction Reagent is the first commercially available yeast lysis reagent to use a mild detergent lysis procedure to efficiently release functionally active solubilized proteins.

This detergent-based cell lysis buffer eliminates the need to use glass beads or mechanical disruption to break through the thick proteinaceous cell envelope to extract protein Y-PER Reagent is effective for S. cerevisiae and other popular species, making it applicable for use in fusion-tagged protein purification and reporter enzyme assays with these model organisms. The lysis reagent also can be used for genomic and plasmid DNA extraction from yeast.

The standard protocol for protein extraction is easy: simply add an appropriate volume of Y-PER Reagent to pelleted yeast cells, incubate at room temperature for approximay 20 minutes and spin down the debris. The resulting supernatant is a concentrated protein solution, surpassing typical yields obtained with traditional glass bead disruption.

Highlights:

• More protein – extracts more than twice as much protein as glass bead methods
• Less hassle – eliminates the difficulties associated with traditional glass bead lysis (e.g., clinging static-charged beads, protein-bead clumps and runaway beads)
• Optmized for yeast – validated for use with Saccharomyces cerevisiae, Schizosaccharomyces pombe and Pichia pastoris
• Versatile – effective for many different organisms, including Bacillus subtilus and both Gram-positive and Gram-negative bacteria; suitable for use in a diverse range of situations