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T-PER (R) Tissue Protein  Extraction Reagent, 500 mL 介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

T-PER (R) Tissue Protein  Extraction Reagent, 500 mL 現(xiàn)貨供應(yīng)

T-PER (R) Tissue Protein  Extraction Reagent, 500 mL 品牌介紹

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主要產(chǎn)品包括：

1)化學(xué)發(fā)光體和比色底物

2)分析試劑

3)熒光標記探針

4)酶聯(lián)免疫分析試劑盒等

T-PER (R) Tissue Protein  Extraction Reagent, 500 mL 僅用于科研

Thermo Scientific T-PER Tissue Protein Extraction Reagent was developed for mild extraction of total protein from tissue samples. This tissue cell lysis reagent utilizes a proprietary detergent in 25mM bicine, 150mM sodium chloride (pH 7.6) to maximize the efficiency of protein solubilization from mammalian tissue samples by homogenization. The simple composition is compatible with additives such as protease inhibitors, salts, reducing agents, chelating agents, providing versatility for many different sample types and lysis applications. Cell lysates prepared with T-PER Reagent are directly compatible with reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures.

Highlights:

• Simple procedure – just homogenize tissue sample in 1:20 (w/v) of tissue to T-PER Reagent, then centrifuge to pellet cell or tissue debris
• Mild and convenient – detergent is dialyzable for quick and easy removal; can be used with additional components (e.g., protease inhibitors, salts, reducing agents, chelating agents, etc.)
• Flexible – lysate may be used for reporter assays, protein kinase assays, immunoassays and/or protein purifications
• Compatible – the lysate is compatible with standard protein assays such as Thermo Scientific Pierce Coomassie Plus (Bradford) Protein Assay