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M-PER (R) Mammalian Protein   Extraction Reagent, 250 mL 介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

M-PER (R) Mammalian Protein   Extraction Reagent, 250 mL 現(xiàn)貨供應(yīng)

M-PER (R) Mammalian Protein   Extraction Reagent, 250 mL 品牌介紹

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主要產(chǎn)品包括：

1)化學(xué)發(fā)光體和比色底物

2)分析試劑

3)熒光標(biāo)記探針

4)酶聯(lián)免疫分析試劑盒等

M-PER (R) Mammalian Protein   Extraction Reagent, 250 mL 僅用于科研

Thermo Scientific M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells. The complete cell lysis reagent is a nondenaturing detergent formulation that dissolves cell membranes and extracts total cellular protein in only 5 minutes. M-PER Reagent requires little or no mechanical disruption and yields more protein than freeze/thaw cycles and sonication. This mammalian cell lysis reagent is so efficient that adherent cells do not need to be scraped from the culture dish, enabling easy and direct lysis and analysis of cells grown in 24-well and 96-well plates. Resulting cell lysates from either adherent and suspension cells are compatible with many downstream assays including immunoassays, enzyme assays and a variety of common reporter assays.

Highlights:

• Gentle – mild detergent lysis, yielding extracts that are immediay compatible with coomassie (Bradford) and BCA protein assays or SDS-PAGE
• Compatible – extracts soluble proteins in nondenatured state, enabling direct use in immunoprecipitation and other affinity purification procedures
• Amine-free and dialyzable – formulation ensures compatibility with subsequent assay systems
• Convenient – lyse adherent cells directly in plate or after scraping and washing in suspension
• Non-denaturing – maintain activity of luciferase, beta-galactosidase, CAT and other reporter genes as well or better than other suppliers' products and freeze/thaw methods