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The B-PER 6xHis Fusion Protein Column and Spin Purification Kits offer rapid purification of His-tagged fusion proteins from bacteria or baculovirus-infected insect cells. The protocol that accompanies the product has been optimized to give a high yield of pure histidine-tagged fusion protein in the least amount of time.

Highlights:

• Capacity
  • purify >10 mg of overexpressed His-tagged fusion protein with the gravity column kit
  • purify >1 mg of overexpressed His-tagged fusion protein with the spin column kit
• Purity – yields histidine-tagged fusion protein free of cellular contamination (Figure 1)
• Speed – progress from lysis to purified protein in less than two hours (30 min with spin kit)
• Easy and efficient cell lysis – no sonication required; complete cell lysis achieved with B-PER Bacterial Protein Extraction Reagent
• Ready-to-use columns and reagents – pre-packed columns (contain 1 ml nickel-chelate agarose), prepared wash and elution buffers make set-up and execution very easy
• Convenient size – kits are designed for single or multiple purifications