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Thermo Scientific Imject Mariculture KLH and the accompanying EDC Conjugation Kit are packages of purified keyhole limpet hemocyanin carrier protein that enable simple preparation of highly effective immunogens with peptide antigens.

KLH is the most popular and immunogenic carrier protein used for preparation of peptide antigens for immunization and antibody production. KLH preparations in phosphate buffer are ready for hapten-carrier conjugation via amine-reactive NHS-ester or glutaraldehyde crosslinking. Preparations in MES buffer are optimized for peptide-carrier conjugation via the carboxyl-reactive carbodiimide crosslinker called EDC (or EDAC). The Imject EDC Spin Kit includes ready-to-use units of KLH carrier protein, optimized reaction buffers and trouble-free spin desalting columns for immunogen preparation using the EDC method.

Highlights:

• Convenient kit – contains lyophilized mcKLH (in MES buffer), EDC crosslinker and accessories components to easily prepare ready-to-use immunogens for injection
• High-yield conjugation – each molecule of KLH contains hundreds of primary amines available for coupling haptens via EDC or NHS ester crosslinkers
• Validated quality – purified and stabilized mariculture KLH maintains solubility in aqueous solutions, unlike traditional sources of the carrier protein
• Sustainable source – KLH is harvested from select populations of the mollusk Megathura crenulata (keyhole limpet) that are grown in mariculture, not extracted from wild populations
• Highly immunogenic – KLH has a high molecular mass (4.5 x 10⁵ to 1.3 x 10⁷ Daltons; aggregates of 350 and 390 kDa subunits) and elicits a stronger immune response than BSA or ovalbumin