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N-HYDROXYSULFOSUCCINIMIDE（SULFO-NHS）介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

N-HYDROXYSULFOSUCCINIMIDE（SULFO-NHS）現(xiàn)貨供應

N-HYDROXYSULFOSUCCINIMIDE（SULFO-NHS）品牌介紹

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主要產(chǎn)品包括：

1）化學發(fā)光體和比色底物

2）分析試劑

3）熒光標記探針

4）酶聯(lián)免疫分析試劑盒等

N-HYDROXYSULFOSUCCINIMIDE（SULFO-NHS）僅用于科研

Thermo Scientific Pierce Sulfo-NHS （N-hydroxysulfosuccinimide） and its uncharged analog NHS （N-hydroxysuccinimide） are chemical modification reagents for converting carboxyl groups to amine-reactive Sulfo-NHS esters for application in a variety of bioconjugation and crosslinking methods.

Derivatives are easily synthesized by mixing the Sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC （EDAC）. The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.
 

Highlights:

• Efficiency of EDC-mediated coupling is increased in the presence of Sulfo-NHS
• Amine-reactive Sulfo-NHS esters can be created on any carboxyl-containing molecule
• Sulfo-NHS derivatives are water-soluble, so crosslinking can be done in physiologic solutions
• High-purity, crystalline Sulfo-NHS can be used to create high-purity activated derivatives