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DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL（DTBP）現(xiàn)貨供應(yīng)

DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL（DTBP）品牌介紹

美國(guó)Pierce公司（www.pierce。。ｃｏｍ）是蛋白質(zhì)化學(xué)、免疫學(xué)和層析等很多方面的和*。http://www.thermo。。ｃｏｍ.cn/Category828.html

主要產(chǎn)品包括：

1）化學(xué)發(fā)光體和比色底物

2）分析試劑

3）熒光標(biāo)記探針

4）酶聯(lián)免疫分析試劑盒等

DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL（DTBP）

僅用于科研

Thermo Scientific Pierce DTBP is dimethyl 3,3'-dithiobispropionimidate, a crosslinker that contains amine-reactive imidoester groups around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

Dimethyl dithiobispropionimidate （DTBP） is a homobifunctional, cleavable and membrane permeable cross-linker. It contains an amine-reactive imidoester at each end of an 8-atom spacer arm. Imidoesters react with primary amines at pH 8-10 to form stable amidine bonds. The amidine is protonated and therefore, like the lysine it modified, has a positive charge at physiological pH. Proteins, including antibodies, generally have several primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for imidoester crosslinking reagents.

DTBP crosslinks can be easily reversed by the addition of 100-150 mM DTT at 37°C for 30 minutes. DMS, the non-cleavable analog of DTBP is available for applications that require a stable, non-cleavable bond. DTSSP, the NHS-ester analog of DTBP is also available for applications that require crosslinking at physiologic pH or greater stability of the crosslinked product at elevated pH. DTBP and DMS have essentially identical crosslinking activity toward primary amines.

DTBP Highlights

• Amine-reactive imidoester reacts rapidly with any primary amine-containing molecule to form amidine bonds
• DTBP crosslinker is water soluble for compatibility with aqueous solutions
• DTBP crosslinking can be easily reversed with DTT, TCEP or other reducing agents
• High purity DTBP can be used to create high-purity conjugates

• High purity DTBP can be used to create high-purity conjugates

DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL（DTBP）介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。