| CBP71015 | |
| I. Background | |
ADORA1 is a receptor for adenosine. It is demonstrated that the ADORA1 receptor is ubiquitous throughout the entire body. Activation of ADORA1 elicits an inhibition of adenylate cyclase and therefore a decrease in the cAMP concentration. ADORA1 receptors are implicated in sleep promotion by inhibiting wake promoting cholinergic neurons in the basal forebrain. Adenosine antagonists are widely used in neonatal medicine. | |
| II. Introduction | |
| Host Cell: | CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10% FBS+200 μg/ml Zeocin+200 μg/ml Hygromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): | Functional assay for A1 receptor |
| Transducer: | Gi |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
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