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詳細(xì)介紹
| 名稱 | 9L/lacZ (大鼠膠質(zhì)肉瘤細(xì)胞) (種屬鑒定正確) |
| 別稱 | 9L/LacZ |
| 種屬 | 大鼠 |
| 年齡（性別） | 雄性 |
| 組織來(lái)源 | 腦，膠質(zhì)細(xì)胞 |
| 生長(zhǎng)特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 成纖維細(xì)胞樣 |
| 背景描述 | The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified. Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide. The contrast between stained cells and background facilitates image analysis. The beta-gal expression is very stable, but cells may need to be re-cloned after months of growth in culture. |
| 生物安全等級(jí) | 1 |
| 生長(zhǎng)培養(yǎng)基 | DMEM(PM150210)＋10%FBS(164210-500)＋1%P/S(PB180120) |
| 推薦傳代比例 | 1:4-1:8 |
| 推薦換液頻率 | 2~3次/周 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度：液氮 |
| 培養(yǎng)條件 | 氣相：空氣，95%；CO2，5%溫度：37℃ |
| 致瘤性 | Yes, forms tumors in the brains of CD Fischer 344 rats |
| 基因表達(dá)情況 | beta galactosidase (beta-gal) |
| 保藏機(jī)構(gòu) | ATCC; CRL-2200 |
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